NEP  (Synonyms: VDP-green (NEP))

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λ_ex/λ_em=430/535 nm). NEP has the potential for parkinsonism^[1].

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells^[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)^[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

654.63

C₂₉H₃₁AsN₄O₅S₂

2414276-32-5

535

430

O=C(N1CCCC)C2=C3C(C(NC(OCC4S[As](SC4)C5=CC=C(C=C5)NC(CCN)=O)=O)=CC=C3C1=O)=CC=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Guodong Hu, et al. Decrease of Protein Vicinal Dithiols in Parkinsonism Disclosed by a Monoarsenical Fluorescent Probe. Anal Chem. 2020 Mar 17;92(6):4371-4378.  [Content Brief]