Nothofagin 

Nothofagin, a dihydrochalcone, is isolated from rooibos (Aspalathus linearis)^[1]. Nothofagin downregulates NF-κB translocation through blocking calcium influx. Nothofagin has antioxidant activity and ameliorates various inflammatory responses such as the septic response and vascular inflammation^[2].

Nothofagin pre-treatment (0.1, 1, 10 μM) decreases the level of histamine release in RBL-2H3 and RPMCs cells. The production of cytokines are downregulated bynothofagin pre-treatment Nothofagin (TNF-α: 1-10 μM; IL-4: 0.1-10 μM, IL-6: 1-10 μM)^[1].
Pre-treatment of DNPHSA-stimulated RBL-2H3 with Nothofagin (10 μM) markedly suppresses the phosphorylation of Lyn, Syk, and Akt^[1].
Nothofagin (30 μM; for 6 hours) results in inhibited formation of LPS-induced (100 ng/mL; 4 hours) paracellular gaps with the formation of dense F-actin rings in HUVECs^[2].
Nothofagin suppresses IgE-mediated mast cell degranulation both in vitro and in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nothofagin (1 mg/kg; orally; once a day; for 7 days) significantly increases the urinary volume of both normotensive (NTR) and spontaneously hypertensive rats (SHR)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

436.41

C₂₁H₂₄O₁₀

11023-94-2

Solid

White to off-white

OC1=C([C@@H]([C@@H]([C@H]2O)O)O[C@@H]([C@H]2O)CO)C(O)=CC(O)=C1C(CCC(C=C3)=CC=C3O)=O

Room temperature in continental US; may vary elsewhere.

• [1]. Wonhwa Lee, et al. Anti-inflammatory Effects of Aspalathin and Nothofagin From Rooibos (Aspalathus Linearis) In Vitro and In Vivo. Inflammation. 2015 Aug;38(4):1502-16.  [Content Brief]

  [2]. Byeong-Cheol Kang, et al. Nothofagin Suppresses Mast Cell-Mediated Allergic Inflammation. Chem Biol Interact. 2019 Jan 25;298:1-7.  [Content Brief]

  [3]. Camila Leandra Bueno de Almeida, et al. Prolonged Diuretic and Saluretic Effect of Nothofagin Isolated From Leandra Dasytricha (A. Gray) Cogn. Leaves in Normotensive and Hypertensive Rats: Role of Antioxidant System and Renal Protection. Chem Biol Interac  [Content Brief]