Nudol

Cat. No.: HY-N7385: FAQs
Data Sheet Handling Instructions

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Nudol is a phenanthrene compound that has anti-cancer activity. Nudol inhibits cell proliferation, induces cell apoptosis. Nudol inhibits MMP-2M and MMP-9 activity (K_i: 988.9 nM, 1.76 μM, respectively). Nudol can be used in the research of cancers, such as osteosarcoma.

For research use only. We do not sell to patients.

Nudol Chemical Structure

CAS No. : 86630-46-8

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•Related Small Molecules:

•Related Proteins:

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View All Isoforms

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

MMP-2

988.9 nM (Ki)

MMP-9

1.76 μM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 15	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 6 days by MTT assay Cytotoxicity against human A549 cells assessed as decrease in cell viability after 6 days by MTT assay	[PMID: 27310249]
HeLa	IC₅₀	> 10 μM Compound: 15	Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 6 days by MTT assay Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 6 days by MTT assay	[PMID: 27310249]
HSC-T6	IC₅₀	> 100 μM Compound: 13	Antiproliferative activity against rat HSC-T6 cells after 48 hrs by MTT assay Antiproliferative activity against rat HSC-T6 cells after 48 hrs by MTT assay	[PMID: 18052323]
MCF7	IC₅₀	> 10 μM Compound: 15	Cytotoxicity against human MCF7 cells assessed as decrease in cell viability after 6 days by MTT assay Cytotoxicity against human MCF7 cells assessed as decrease in cell viability after 6 days by MTT assay	[PMID: 27310249]
Neutrophil	IC₅₀	> 10 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as elastase substrate preincubated for 5 mins followed by fMLP/CB-induction Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as elastase substrate preincubated for 5 mins followed by fMLP/CB-induction	[PMID: 27525452]
Neutrophil	IC₅₀	1.7 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/cytochalasin B-induced superoxide anion generation by measuring superoxide dismutase-inhibitable reduction of ferricytochrome c preincubated for 5 mins followed by FMLP/cytochal Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/cytochalasin B-induced superoxide anion generation by measuring superoxide dismutase-inhibitable reduction of ferricytochrome c preincubated for 5 mins followed by FMLP/cytochal	[PMID: 27525452]

In Vitro

Nudol (0-40 μM, 24-72 h) decreases cell viability in several cancer cell lines^[1].
Nudol (0-20 μM, 24/48 h) suppresses the cell migration and causes cell cycle arrest at G2/M phase in U2OS cells^[1].
Nudol (20 μM, 48 h) induces cell apoptosis through the caspase-dependent pathway in U2OS cells^[1].
Nudol (compound 4) inhibits MMP-2 and MMP-9 activity with K_i values of 988.9 nM and 1.76 μM respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	U2OS, MG63, MDA-MB-231, MCF-7, and A549 cells
Concentration:	0, 10, 20, 30, 40 μM
Incubation Time:	24, 48, and 72 h
Result:	Decreased the cell viability of osteosarcoma cells in dose- and time-dependent manners.

Cell Cycle Analysis^[1]

Cell Line:	U2OS cells
Concentration:	0, 5, 10, 20 μM
Incubation Time:	24, 48, and 72 h
Result:	Triggered G2/M phase arrest by decreasing cell cycle-related proteins (CDK1, CDK2, CDK4, and CDK10).

Western Blot Analysis^[1]

Cell Line:	U2OS cells
Concentration:	0, 5, 10, 20, 40 μM
Incubation Time:	24, 48, and 72 h
Result:	Increased the protein level of cytochrome c. Down-regulated anti-apoptotic Bcl-2, accompanied by an increased level of pro-apoptotic Bax.

Molecular Weight

270.28

Formula

C₁₆H₁₄O₄

CAS No.

86630-46-8

SMILES

OC1=C(OC)C(OC)=C2C3=CC=C(O)C=C3C=CC2=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Yuying Zhang, et al. Nudol, a phenanthrene derivative from Dendrobium nobile, induces cell cycle arrest and apoptosis and inhibits migration in osteosarcoma cells. Drug Des Devel Ther. 2019 Jul 29;13:2591-2601. [Content Brief]

[2]. Mohammad Al-Amin, et al. Inhibitory Effect of Dioscorea bulbifera Tubers on the Migration of Triple-Negative Breast Cancer Cells. Breast Cancer Cells. Rev. Bras. Farmacogn. 31, 335–341 (2021).

Nudol Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nudol86630-46-8MMPApoptosisMatrix metalloproteinasesAnti-proliferationphenanthreneanti-tumoranti-cancerosteosarcomaU2OSapoptosisInhibitorinhibitorinhibit

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