1. Immunology/Inflammation Anti-infection
  2. Toll-like Receptor (TLR) Bacterial
  3. ODN 1668

ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’.

For research use only. We do not sell to patients.

DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)

ODN 1668 Chemical Structure

CAS No. : 1186063-66-0

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Based on 1 publication(s) in Google Scholar

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Description

ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’[1][2][3][4].

IC50 & Target[4]

TLR9

 

In Vitro

ODN 1668 (6-60 µg/mL; 12-72 h) increases the synthesis of S100A8/A9, IL-6 and β-galactosidase in mouse bone marrow derived macrophages[1].
ODN 1668 (24 h) promotes the activation and proliferation of B cells and increases the expression of CD86 and CD25[2].
ODN 1668 (0.3-3.0 μM; 4 days) promotes cell growth in anti-CD3-treated CD8+ T cells in a dose-dependent manner[2].
ODN 1668 (0.25-32 μg/mL; 12 h) promotes the proliferation of head kidney lymphocytes and enhances the expression of immune genes[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR[3]

Cell Line: Head kidney lymphocytes
Concentration: 2 μg/mL
Incubation Time: 12 h
Result: Increased the mRNA levels of TLR9, IL-1β, IL-6, IL-8, TNF-α, IFN-γ, MHC-Iα and MHC-IIα.
In Vivo

ODN 1668 (10 nmol ≈ 63.6 μg; injection into the hind footpads; single dose) significantly enhances antibody response in OVA treated mice and increases titers of IgG2a and IgG2b[2].
ODN 1668 (15 μg; intramuscular injection; single dose) improves the antibacterial immunity of Epinephelus laminatus infection, and has the most significant antibacterial activity in liver, spleen and kidney[3].
ODN 1668 (1-5 mg/kg; intraperitoneal/subcutaneous injection; single dose) can cause fever, anorexia and brain inflammation in rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (175-200 g)[4]
Dosage: 1 and 5 mg/kg
Administration: Intraperitoneal and subcutaneous injection, single dose
Result: Caused moderate fever and anorexia.
Induced a significant increase of IL-6.
Increased expression of inflammatory genes in the hypothalamus (SOCS3, PGE2, COX-2 and mPGES).
Activated inflammatory transcription factors in the rat brain (STAT3 and NF-IL6).
Molecular Weight

6364.10

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)

SMILES

[DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 20 mg/mL (3.14 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.1571 mL 0.7857 mL 1.5713 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.1571 mL 0.7857 mL 1.5713 mL 3.9283 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ODN 1668
Cat. No.:
HY-150726
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