2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. ODN 2007

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor (TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (ODN 2007 sodium) that retains the same biological activity.

For research use only. We do not sell to patients.

DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)

ODN 2007 Chemical Structure

CAS No. : 455348-63-7

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Other Forms of ODN 2007:

ODN 2007 Related Antibodies

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Description

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor (TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'[1][2][3].

In Vitro

ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression[1].
ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ODN 2007 Related Antibodies

RT-PCR[1]

Cell Line: MQ-NCSU cells (a chicken macrophage cell line)
Concentration: 1 μg/mL, 5 μg/mL
Incubation Time: 3 h, 12 h, 18 h
Result: Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose.
Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point.

Western Blot Analysis[3]

Cell Line: HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line
Concentration: 10 μg/mL
Incubation Time: 0.5 h, 1 h, 3 h, 6 h, 12 h
Result: Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels.
Stimulated an increase in the level of NO.
In Vivo

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticus FJ03-X2[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish infected with Vibrio traumaticus FJ03-X2[2]
Dosage: 1 μg
Administration: Intraperitoneal injection; once
Result: Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%.
Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.
Molecular Weight

6852.50

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)
SMILES

[DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 20 mg/mL (2.92 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.1459 mL 0.7297 mL 1.4593 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.1459 mL 0.7297 mL 1.4593 mL 3.6483 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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ODN 2007 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ODN 2007455348-63-7ODN2007ODN-2007Toll-like Receptor (TLR)oligodeoxynucleotideToll-like receptor ligandimmunomodulatorvaccine adjuvantInhibitorinhibitorinhibit

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