OXSI-2

Cat. No.: HY-112386: FAQs
Data Sheet Handling Instructions

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OXSI-2 is a bioavailable, cell-permeable Syk inhibitor with an EC₅₀ of 313 nM and an IC₅₀ of 14 nM.

For research use only. We do not sell to patients.

OXSI-2 Chemical Structure

CAS No. : 622387-85-3

Size	Price	Stock	Quantity
500 μg	USD 66	In-stock	Estimated Time of Arrival: December 31
1 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
5 mg		Get quote
10 mg		Get quote

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Purity & Documentation
References
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Description

OXSI-2 is a bioavailable, cell-permeable Syk inhibitor with an EC₅₀ of 313 nM and an IC₅₀ of 14 nM^[1]^[2].

In Vitro

OXSI-2 (2 μM) completely inhibits Convulxin-induced platelet aggregation and shape change. OXSI-2 (2 μM) also completely blocks GPVI-mediated dense granule release. OXSI-2 (100 nM) does not affect the platelet functional responses induced by Convulxin, and modest shape change is still evident at 1 μM^[1].
Adaptor protein LAT is a known substrate of Syk Kinase. OXSI-2 completely inhibits LAT Y191 phosphorylation. OXSI-2 inhibits Syk mediated events in platelets^[1].
OXSI-2 (2 μM) inhibits inflammasome assembly, caspase-1 activation, IL-1β processing and release, mitochondrial ROS generation, and pyroptotic cell death^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Aspirin-treated, washed human platelets
Concentration:	2 μM
Incubation Time:
Result:	Completely inhibited Syk-mediated LAT Y191 phosphorylation.

Molecular Weight

353.40

Formula

C₁₈H₁₅N₃O₃S

CAS No.

622387-85-3

Appearance

Solid

Color

Light yellow to brown

SMILES

O=S(C1=CC2=C(NC(/C2=C/C3=CN(C)C4=C3C=CC=C4)=O)C=C1)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (28.30 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8297 mL	14.1483 mL	28.2965 mL
5 mM	0.5659 mL	2.8297 mL	5.6593 mL
10 mM	0.2830 mL	1.4148 mL	2.8297 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Kamala Bhavaraju, et al. Evaluation of [3-(1-methyl-1H-indol-3-yl-methylene)-2-oxo-2, 3-dihydro-1H-indole-5-sulfonamide] (OXSI-2), as a Syk-selective inhibitor in platelets. Eur J Pharmacol. 2008 Feb 12;580(3):285-90. [Content Brief]

[2]. Jordan R Yaron, et al. The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium efflux. Biochem Biophys Res Commun. 2016 Apr 8;472(3):545-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8297 mL	14.1483 mL	28.2965 mL	70.7414 mL
	5 mM	0.5659 mL	2.8297 mL	5.6593 mL	14.1483 mL
	10 mM	0.2830 mL	1.4148 mL	2.8297 mL	7.0741 mL
	15 mM	0.1886 mL	0.9432 mL	1.8864 mL	4.7161 mL
	20 mM	0.1415 mL	0.7074 mL	1.4148 mL	3.5371 mL
	25 mM	0.1132 mL	0.5659 mL	1.1319 mL	2.8297 mL

OXSI-2 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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