p-Nitrophenyl α-L-arabinopyranoside 

p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection^[1][3].

The affinity K_m value of p-Nitrophenyl α-L-arabinopyranoside with rBgaA is 6.06 mM^[1].
p-Nitrophenyl α-L-arabinopyranoside shows high activity to xylosidase–arabinosidase (xarB) gene^[2].
Application of p-Nitrophenyl α-L-arabinopyranoside in the detection of enzyme activity^[3]
(1) 200 µL 2 mM p-Nitrophenyl α- L-arabinopyranoside, 100 µL enzyme, 300 µL 50 mM phosphate buffer (pH 7.0) were incubated at 37℃ for 0.5, 1, and 5 h.
(2) 400 µL 0.5 M NaOH was added to terminate the reaction.
(3) Measure the absorbance of the mixture at 405 nm.
Note: In the presence of ginsenosides, the reaction was terminated by extraction with butanol.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

271.22

C₁₁H₁₃NO₇

1223-07-0

O[C@H]([C@H]([C@H](CO1)O)O)[C@@H]1OC2=CC=C([N+]([O-])=O)C=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kosugi A, et al. Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002 Dec;184(24):6859-65.  [Content Brief]

  [2]. Mai V, et al. Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus. Gene. 2000 Apr 18;247(1-2):137-43.  [Content Brief]

  [3]. Shin H Y, et al. Purification and Characterization ofα-l-Arabinopyranosidase andα-l-Arabinofuranosidase from Bifidobacterium breve K-110, a Human Intestinal Anaerobic Bacterium Metabolizing Ginsenoside Rb2 and Rc[J]. Applied and environmental microbiology, 2003, 69(12): 7116-7123.