Peroxidase, Horseradish

Name: Peroxidase, Horseradish
Brand: MedChemExpress
SKU: HY-125859
Rating: 4.5 (1 reviews)

Cat. No.: HY-125859: FAQs
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Peroxidase, Horseradish actively involves in oxidizing reactive oxygen species, innate immunity, hormone biosynthesis and pathogenesis of several diseases.

For research use only. We do not sell to patients.

Peroxidase, Horseradish Chemical Structure

CAS No. : 9003-99-0

Size	Price	Stock	Quantity
10 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 59	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 94	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 525	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Peroxidase, Horseradish

•Redox Biol. 2023 Sep;65:102837. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Peroxidase, Horseradish actively involves in oxidizing reactive oxygen species, innate immunity, hormone biosynthesis and pathogenesis of several diseases^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.9 μM Compound: 8, MGCD0103	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 18570366]
A549	IC₅₀	1.279 μM Compound: MGCD0103	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23206867]
A549	IC₅₀	1.65 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24095018]
A549	IC₅₀	1.73 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human A549 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human A549 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
A549	IC₅₀	11.87 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
A549	IC₅₀	14.57 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
A549	IC₅₀	2.08 μM Compound: Mocetinostat	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
A549	IC₅₀	59.9 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
CT26	GI₅₀	1.77 μM Compound: 4; MGCD-0103	Antiproliferative activity against mouse CT26 cells after 72 hrs by CCK-8 assay Antiproliferative activity against mouse CT26 cells after 72 hrs by CCK-8 assay	[PMID: 35390714]
CT26	GI₅₀	1.8 μM Compound: 9; MGCD-0103	Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay	[PMID: 34783558]
DU-145	IC₅₀	0.67 μM Compound: 8, MGCD0103	Antiproliferative activity against human Du145 cells after 72 hrs by MTT assay Antiproliferative activity against human Du145 cells after 72 hrs by MTT assay	[PMID: 18570366]
DU-145	IC₅₀	2.06 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human DU145 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human DU145 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
HCT-116	IC₅₀	0.29 μM Compound: 8, MGCD0103	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 18570366]
HCT-116	IC₅₀	0.3 μM Compound: A, MGCD0103	Antiproliferative activity against human HCT116 cells by MTT assay Antiproliferative activity against human HCT116 cells by MTT assay	[PMID: 19114304]
HCT-116	IC₅₀	0.327 μM Compound: MGCD0103	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 23206867]
HCT-116	IC₅₀	0.396 μM Compound: Mocetinostat	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HCT-116	EC₅₀	0.6 μM Compound: A, MGCD0103	Induction of p21WAF1/CIP1 expression in human HCT116 cells assessed as tubulin level after 16 hrs by luciferase assay Induction of p21WAF1/CIP1 expression in human HCT116 cells assessed as tubulin level after 16 hrs by luciferase assay	[PMID: 19114304]
HCT-116	IC₅₀	0.7 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human HCT116 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human HCT116 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
HCT-116	IC₅₀	1.24 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HCT-116	IC₅₀	1.57 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24095018]
HCT-116	IC₅₀	29.69 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
HCT-116	IC₅₀	3.51 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	3.32 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	3.42 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	43.8 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
Hep 3B2	IC₅₀	0.823 μM Compound: Mocetinostat	Cytotoxicity against human Hep3B cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human Hep3B cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HepG2	IC₅₀	0.876 μM Compound: Mocetinostat	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HepG2	IC₅₀	4.05 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HepG2	IC₅₀	4.25 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HepG2	IC₅₀	5.79 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
HMEC	IC₅₀	20 μM Compound: 8, MGCD0103	Antiproliferative activity against HMEC after 72 hrs by MTT assay Antiproliferative activity against HMEC after 72 hrs by MTT assay	[PMID: 18570366]
HMEC	IC₅₀	21 μM Compound: A, MGCD0103	Antiproliferative activity against HMEC by MTT assay Antiproliferative activity against HMEC by MTT assay	[PMID: 19114304]
MC-38	GI₅₀	1.64 μM Compound: 4; MGCD-0103	Antiproliferative activity against mouse MC38 cells after 72 hrs by CCK-8 assay Antiproliferative activity against mouse MC38 cells after 72 hrs by CCK-8 assay	[PMID: 35390714]
MC-38	GI₅₀	21 μM Compound: 9; MGCD-0103	Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay	[PMID: 34783558]
MCF7	IC₅₀	1.26 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human MCF7 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human MCF7 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
MCF7	IC₅₀	2.49 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
MCF7	IC₅₀	4.807 μM Compound: MGCD0103	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 23206867]
MCF7	IC₅₀	56.81 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
MCF7	IC₅₀	84.17 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
MHCC97H	IC₅₀	4.563 μM Compound: Mocetinostat	Cytotoxicity against human MHCC97H cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MHCC97H cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
MKN-45	IC₅₀	0.61 μM Compound: Mocetinostat	Cytotoxicity against human MKN45 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MKN45 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
PANC-1	IC₅₀	26.774 μM Compound: Mocetinostat	Cytotoxicity against human PANC1 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human PANC1 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
Sf9	IC₅₀	> 10000 nM Compound: MGCD0103	Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	[PMID: 23009203]
Sf9	IC₅₀	102 nM Compound: MGCD0103	Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	[PMID: 23009203]
SJSA-1	IC₅₀	3.624 μM Compound: Mocetinostat	Cytotoxicity against human SJSA1 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SJSA1 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SNU-16	IC₅₀	0.142 μM Compound: Mocetinostat	Cytotoxicity against human SNU16 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SNU16 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SNU-5	IC₅₀	1.009 μM Compound: Mocetinostat	Cytotoxicity against human SNU5 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SNU5 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SW-620	IC₅₀	0.419 μM Compound: Mocetinostat	Cytotoxicity against human SW620 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SW620 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
T-24	EC₅₀	< 1 μM Compound: A, MGCD0103	Induction of histone H4 hyperacetylation in human T24 cells after 16 hrs by immunoblotting Induction of histone H4 hyperacetylation in human T24 cells after 16 hrs by immunoblotting	[PMID: 19114304]

In Vitro

Peroxidases belong to a large family of isoenzymes present in almost all living organisms. These are generally heme containing enzymes ranging in Mw from 35-100 Kd. Mammalian peroxidases are much larger proteins (576-738 amino acids) than the plant counterparts. Peroxidases exist as monomers, dimmers or tetramers and their gene locations also vary among different chromosomes. Peroxidases have some organ, tissue, cellular and sub-cellular specific distribution patterns, performing some specific functions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.

9003-99-0

Appearance

Solid

Color

Yellow to brown

SMILES

[Peroxidase, Horseradish]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (ultrasonic and warming and heat to 60°C)

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.1M Phosphate Buffer
Solubility: 2 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

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This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

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Purity & Documentation

Select Batch:

Data Sheet (268 KB) SDS (419 KB)

COA (229 KB)

Handling Instructions (2659 KB)

References

[1]. Khan AA, et al. Biochemical and pathological studies on peroxidases -an updated review. Glob J Health Sci. 2014 May 13;6(5):87-98. [Content Brief]