2. Others Metabolic Enzyme/Protease
  3. Biochemical Assay Reagents Glucosidase
  4. PNGase F

PNGase F, a glycosidase, catalyzes the cleavage of an internal glycoside bond in an oligosaccharide. PNGase F removes nearly all N-linked oligosaccharides from glycoproteins. PNGase F can release N-glycans from glycoproteins in glycoanalytical workflows.

For research use only. We do not sell to patients.

PNGase F Chemical Structure

PNGase F Chemical Structure

CAS No. : 83534-39-8

Size Price Stock Quantity
10 KU USD 200 In-stock

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

PNGase F Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE PNGase F

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PNGase F, a glycosidase, catalyzes the cleavage of an internal glycoside bond in an oligosaccharide. PNGase F removes nearly all N-linked oligosaccharides from glycoproteins. PNGase F can release N-glycans from glycoproteins in glycoanalytical workflows[1][2].

In Vitro

Application: 1. In vitro de-glycosylation modification of antibodies, immunoglobulin fusion proteins or other glycoproteins; 2. Characterization of whether there is N-glycosylation modification in proteins.

molecular weigh: 36 kDa
optimal temperature: 37 ℃
pH range:6.0-10.0
temperature range: 4-50 ℃
ionic strength range: 0-1 M NaCl
PNGase F storage solution: 10 m M Tris (pH7.4), 50 m M NaCl, 5 m M EDTA. The storage solution does not contain glycerol, making it easier to optimize chromatographic conditions for HPLC methods.
Reaction Buffer (10X): 500 m M Sodiu M Phosphate (pH7.5)

PNGase F cannot hydrolyze N-glycosides containing core α 1-3 fucose, which are common in plant and insect glycoproteins. In this case, PNGase A needs to be used.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PNGase F Related Antibodies
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[PNGase F]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

COA (242 KB)

Handling Instructions (2659 KB)
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

PNGase F Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PNGase F83534-39-8Biochemical Assay ReagentsGlucosidaseGlycosidaseN-linkedoligosaccharidesN-glycanInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PNGase F
Cat. No.:
HY-P2929
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.