2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. PPAR
  4. PPARγ agonist 17

PPARγ agonist 17 (Compound C1) is a PPARγ agonist. PPARγ agonist 17 enhances PPARγ activity and blocks the cell cycle in G2/M phase, inhibits cell migration and induces apoptosis in HT-29 cells. PPARγ agonist 17 has a broad spectrum anti-proliferative activity in cancer cells with relatively low toxicity in normal cells which cannot cross the blood-brain barrier.

For research use only. We do not sell to patients.

PPARγ agonist 17 Chemical Structure

PPARγ agonist 17 Chemical Structure

CAS No. : 3054652-58-0

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

PPARγ agonist 17 Related Antibodies

Top Publications Citing Use of Products

View All PPAR Isoform Specific Products:

View All Isoforms
PPARα PPARβ/δ PPARγ PPAR PPARδ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PPARγ agonist 17 (Compound C1) is a PPARγ agonist. PPARγ agonist 17 enhances PPARγ activity and blocks the cell cycle in G2/M phase, inhibits cell migration and induces apoptosis in HT-29 cells. PPARγ agonist 17 has a broad spectrum anti-proliferative activity in cancer cells with relatively low toxicity in normal cells which cannot cross the blood-brain barrier[1].

In Vitro

PPARγ agonist 17 (0-1000 μM, 48 h) exhibits good anti-proliferative activity against HCT-116, HT-29, HCT-8, MCF-7, MDA-MB-231, without damaging 293 T and L02[1].
PPARγ agonist 17 (4-8 μM) significantly enhances PPARγ activity in HT-29 cells. PPARγ agonist 17 (2-8 μM, 24 h) significantly reduces the proportion of EdU positive cells, and effectively inhibits the proliferation of HT-29 cells, and blocks the cell cycle in G2/M phase, inhibits the expression of cyclin D1 and CDK4. PPARγ agonist 17 significantly inhibits the number and size of HT-29 cell colony formation in a dose-dependent manner, and induces apoptosis. PPARγ agonist 17 (2-4 μM, 0-48 h) inhibits HT-29 cell migration[1].
PPARγ agonist 17 demonstrates relatively low toxicity, with no tumorigenic effects and low-AMES toxicity (non-mutagenic), as well as comparatively lower acute oral toxicity than 18β-Glycyrrhetinic acid (HY-N0180) and Rosiglitazone (HY-17386, ROS) [1].
PPARγ agonist 17 cannot cross the blood-brain barrier, thus avoiding central nervous system toxicity[1].
PPARγ agonist 17 shows adequate human liver microsomal (HLM) stability and a suitable T1/2, indicating that it has sufficient metabolic stability in human liver microsomes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PPARγ agonist 17 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HT-29
Concentration: 2, 4, 8 μM
Incubation Time: 24 h
Result: Inhibited the number and size of HT-29 cell colony formation in a dose-dependent manner.

Cell Proliferation Assay[1]

Cell Line: HCT-116, HT-29, HCT-8, MCF-7, MDA-MB-231, 293 T, L02
Concentration: 0, 0.1, 1, 10, 100, 1000 μM
Incubation Time: 48 h
Result: Showed anti-proliferative activity against HCT-116, HT-29, HCT-8, MCF-7, MDA-MB-231 with IC50 values of 25.02 μM, 12.25 μM, 33.13 μM, 15.23 μM, 18.58 μM., without damaging 293 T and L02 cells.
Had an anti-proliferative activity against the HT-29 cell line that is 12 times that of its parent skeleton 18β-GA and ROS.

Cell Migration Assay [1]

Cell Line: HT-29
Concentration: 2, 4 μM
Incubation Time: 0, 24, 48 h
Result: Could significantly slow the healing of scratches in a dose-dependent manner, indicating its ability to inhibit the migration of HT-29 cells.

Apoptosis Analysis[1]

Cell Line: HT-29
Concentration: 2, 4, 8 μM
Incubation Time: 24 h
Result: Induced apoptosis of HT-29 cells.

Cell Cycle Analysis[1]

Cell Line: HT-29
Concentration: 2, 4, 8 μM
Incubation Time: 24 h
Result: Could significantly block HT-29 cell cycle in G2/M phase in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: HT-29
Concentration: 2, 4, 8 μM
Incubation Time: 24 h
Result: Significantly inhibited the expression of cyclin D1 and CDK4.
Significantly up-regulated the expression level of proapoptotic protein BAX and down-regulated the expression level of anti-apoptotic protein Bcl2, thereby activating downstream caspase-3, leading to an increase in the levels of cleaved caspase-3 and cytochrome C.

Western Blot Analysis[1]

Cell Line: HT-29
Concentration: 2, 4, μM
Incubation Time: 0, 24, 48 h
Result: Could effectively regulate the expression of the migration-related proteins, such as up-regulating the expression of E-cadherin and down-regulating the expression of β-catenin and vimentin.
Molecular Weight

766.02

Formula

C48H63NO7
CAS No.
SMILES

C[C@]12[C@@](C(C=C3[C@]2(CC[C@@]4([C@@]3([H])C[C@](CC4)(C(O)=O)C)C)C)=O)([H])[C@@]5([C@@](C(C)([C@H](CC5)OC(CC6=CC=CC(NC(COC7=C(C)C=CC(C)=C7)=O)=C6)=O)C)([H])CC1)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

PPARγ agonist 17 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PPARγ agonist 173054652-58-0PPARγ agonist17PPARγ agonist-17PPARPeroxisome proliferator-activated receptorsPPARγapoptosisanti-proliferativeHCT-116HT-29HCT-8MCF-7MDA-MB-231cell cycleantitumorInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PPARγ agonist 17
Cat. No.:
HY-173166
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.