Pseudouridimycin TFA  (Synonyms: PUM TFA)

Pseudouridimycin (PUM) TFA is an antibiotic that selectively inhibits bacterial RNA polymerase (RNAP), with an IC₅₀ of about 0.1 μM and MICs of 4-6 μg/mL. Pseudouridimycin TFA is a C-nucleoside analogue that's effective against both Gram-negative and Gram-positive bacteria. Pseudouridimycin TFA inhibits bacterial growth in vitro and shows activity in a mouse model of purulent streptococcal peritonitis^[1][2].

Pseudouridimycin (PUM) TFA is selective because of its side chain that binds in a region that is conserved in bacterial RNA polymerases but not in humans. In vitro bactericidal activity is reported for Pseudouridimycin TFA against drug-sensitive, drug-resistant, and multidrug-resistant Streptococcus species^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pseudouridimycin (3.2-50 mg/kg; iv; 10 min after infection and again 6 h after infection) TFA clears infection in vivo in a mouse Streptococcus pyogenes peritonitis model (ED₅₀ = 9 mg/kg)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

600.46

C₁₉H₂₇F₃N₈O₁₁

2760807-99-4

O=C1NC(C([C@H]2[C@@H]([C@@H]([C@@H](CNC([C@H](CCC(N)=O)N(C(CNC(N)=N)=O)O)=O)O2)O)O)=CN1)=O.OC(C(F)(F)F)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Rosenqvist P, et al. Characterization of C-nucleoside Antimicrobials from Streptomyces albus DSM 40763: Strepturidin is Pseudouridimycin. Sci Rep. 2019 Jun 20;9(1):8935.  [Content Brief]

  [2]. Kunthavai Pavundurai Chandra, et al. Pseudouridimycin-A Potent Nucleoside Inhibitor of the RNA. ACS Omega. 2023 Feb 13;8(8):7989-8000.  [Content Brief]

  [3]. Maffioli SI, et al. Antibacterial Nucleoside-Analog Inhibitor of Bacterial RNA Polymerase. Cell. 2017 Jun 15;169(7):1240-1248.e23.