2. Recombinant Proteins
  3. AARS1 Protein, Mouse (sf9, His, solution)

The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background

AARS1, a pivotal enzyme in protein synthesis, plays a crucial role in the attachment of alanine to tRNA(Ala) through a two-step process. Initially, alanine undergoes activation by ATP to form Ala-AMP, and subsequently, it is transferred to the acceptor end of tRNA(Ala). Beyond its primary aminoacylation function, AARS1 boasts an editing domain that enables the correction of incorrectly charged tRNA(Ala), underscoring its role in maintaining the fidelity of the translation process. This dual functionality highlights AARS1's significance in ensuring the accuracy and integrity of the protein synthesis machinery.
Species

Mouse
Source

Sf9 insect cells
Tag

C-His
Accession

Q8BGQ7 (M1-N968)
Gene ID

234734
Molecular Construction
N-term
AARS1 (M1-N968)
Accession # Q8BGQ7
His
C-term
Synonyms
Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; AlaRS; Sti; Aars
AA Sequence

MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAA

Molecular Weight

Approximately 108.43 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation

COA (206 KB)

Handling Instructions (2659 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AARS1 Protein, Mouse (sf9, His, solution)
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