ABHD4 Protein, Human (sf9, His)

ABHD4 is a lysophospholipase that acts selectively on N-acylphosphatidylethanolamines (NAPE), which is essential for the biosynthesis of N-acylphosphatidylethanolamines such as anandamide. It hydrolyzes the sn-1 and sn-2 acyl chains in NAPE to generate glycerophosphate-N-acylethanolamine (GP-NAE), which is an important intermediate in the production of N-acylethanolamine. ABHD4 Protein, Human (sf9, His) is the recombinant human-derived ABHD4 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ABHD4 Protein, Human (sf9, His) is 342 a.a., with molecular weight of ~40 KDa.

ABHD4, a lysophospholipase, exhibits selectivity for N-acyl phosphatidylethanolamine (NAPE) and plays a vital role in the biosynthesis of N-acyl ethanolamines, such as the endocannabinoid anandamide. It achieves this by hydrolyzing the sn-1 and sn-2 acyl chains from NAPE, generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate crucial for N-acyl ethanolamine biosynthesis. ABHD4 demonstrates activity across a range of substrates with saturated, monounsaturated, and polyunsaturated N-acyl chains, contributing to the diversity of N-acyl ethanolamines produced. Notably, it exhibits no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.

Human

Sf9 insect cells

N-His

Q8TB40 (M1-D342)

63874  [NCBI]

Approximately 40 kDa.

Greater than 82% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.