2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. ACAA1 Protein, Human (sf9, His)

ACAA1 Protein, Human (sf9, His)

Cat. No.: HY-P75558
Handling Instructions

The ACAA1 protein catalyzes the thiolytic cleavage of various straight-chain 3-keto fatty acyl-CoAs, playing a vital role in peroxisomal beta-oxidation of short to very long 3-oxoacyl-CoAs. ACAA1 Protein, Human (sf9, His) is the recombinant human-derived ACAA1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ACAA1 Protein, Human (sf9, His) is 398 a.a., with molecular weight of ~43.8 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The ACAA1 protein catalyzes the thiolytic cleavage of various straight-chain 3-keto fatty acyl-CoAs, playing a vital role in peroxisomal beta-oxidation of short to very long 3-oxoacyl-CoAs. ACAA1 Protein, Human (sf9, His) is the recombinant human-derived ACAA1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ACAA1 Protein, Human (sf9, His) is 398 a.a., with molecular weight of ~43.8 kDa.
Background

The ACAA1 protein is responsible for the thiolytic cleavage of straight-chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs). It plays a crucial role in fatty acid peroxisomal beta-oxidation, exhibiting catalytic activity in the cleavage of short, medium, long, and very long straight-chain 3-oxoacyl-CoAs.
Species

Human
Source

Sf9 insect cells
Tag

N-His
Accession

P09110 (L27-N424)
Gene ID

30  [NCBI]
Molecular Construction
N-term
His
ACAA1 (L27-N424)
Accession # P09110
C-term
Synonyms
3-ketoacyl-CoA thiolase, peroxisomal; Beta-ketothiolase; ACAA1; ACAA; PTHIO
Molecular Weight

Approximately 43.8 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

ACAA1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ACAA1 Protein, Human (sf9, His)
Cat. No.:
HY-P75558
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.