ACAT2 Protein, Human (Trx-His)

ACAT2, Human (Trx-His) is the major ACAT enzyme in human hepatocytes. ACAT2, Human is an important regulator of susceptibility to the development of atherosclerosis.

ACAT2 is the major ACAT enzyme in human hepatocytes. Currently, two ACAT isozymes, ACAT1 and ACAT2, have been identified in humans and in animals. ACAT2 expression predominantly in the liver and intestine. ACAT2 is the major isoenzyme in monkey and mouse hepatocytes. ACAT1 is the major isoform in human hepatocytes. ACAT2 is identified as an important regulator of susceptibility to the development of atherosclerosis. Studies in mouse models of atherosclerosis showed reduced amounts of atherosclerosis when ACAT2 was deficient^[1][2].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-Trx;N-6*His

Q9BWD1 (M1-E397)

39  [NCBI]

MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Parini P, et, al. ACAT2 is localized to hepatocytes and is the major cholesterol-esterifying enzyme in human liver. Circulation. 2004 Oct 5;110(14):2017-23.  [Content Brief]

  [2]. Sakashita N, et, al. Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) is induced in monocyte-derived macrophages: in vivo and in vitro studies. Lab Invest. 2003 Nov;83(11):1569-81.  [Content Brief]