Agrin Protein, Rat (HEK293, His)

Agrin, especially the neuron-specific (z+) isoform, plays a crucial role in neuromuscular junction (NMJ) formation and postsynaptic differentiation. It activates the AGRN-LRP4 receptor complex, leading to NMJ formation through phosphorylation of MUSK. Agrin Protein, Rat (HEK293, His) is the recombinant rat-derived Agrin protein, expressed by HEK293 , with N-6*His labeled tag and P1788-S1798 del.

Agrin protein is a heparan sulfate basal lamina glycoprotein that has a crucial role in the formation and maintenance of the neuromuscular junction (NMJ), as well as in directing key events in postsynaptic differentiation. Specifically, the neuron-specific (z+) isoform of Agrin is a component of the AGRN-LRP4 receptor complex, which activates and phosphorylates MUSK, thereby inducing NMJ formation. This process involves the regulation of various processes, including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane, with calcium ions playing a critical role in AChR clustering. The neuronal function of Agrin is tightly regulated by alternative splicing, glycan binding, and proteolytic processing. In addition to its role in NMJ formation, Agrin also modulates calcium ion homeostasis in neurons by increasing cytoplasmic calcium ion levels. In the central nervous system (CNS), Agrin functions by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and inducing depolarization at CNS synapses. The transmembrane isoform of Agrin, known as TM-agrin, is predominantly expressed in brain neurons and promotes dendritic filopodia and synapse formation in mature hippocampal neurons, primarily through the action of attached glycosaminoglycan chains and Rho-family GTPases. Multiple isoforms of Agrin, including isoform 1, isoform 4, isoform 5, and isoform 6, contain neuron-specific C-terminal insertions and are potent activators of AChR clustering. Isoform 5, known as agrin (z+8), which contains an 8-AA insertion, forms a receptor complex with the neuronal AGRN, muscle-specific kinase MUSK, and LRP4. The splicing factors NOVA1 and NOVA2 regulate the splicing of Agrin and the production of the 'z' isoforms.

Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 0.3128 μg/mL, corresponding to a specific activity is 3.197×10³ units/mg.

Rat

HEK293

N-6*His

P25304-1 (A1153-P1959, P1788-S1798 del)

25592

AATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Approximately 95-97 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS , pH 6.8.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.