Alanyl-tRNA synthetase Protein, Human (sf9, His)

The Alanyl-tRNA synthetase protein facilitates a two-step process, activating alanine with ATP to form Ala-AMP and transferring it to the acceptor end of tRNA(Ala). Additionally, it corrects incorrectly charged tRNA(Ala) through its editing domain. Alanyl-tRNA synthetase Protein, Human (sf9, His) is the recombinant human-derived Alanyl-tRNA synthetase protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Alanyl-tRNA synthetase Protein, Human (sf9, His) is 968 a.a., with molecular weight of ~109.2 kDa.

Alanyl-tRNA synthetase, a key player in protein synthesis, orchestrates the attachment of alanine to tRNA(Ala) through a meticulous two-step process. Initially, alanine undergoes activation by ATP, leading to the formation of Ala-AMP. Subsequently, this activated alanine is diligently transferred to the acceptor end of tRNA(Ala). Beyond its primary aminoacylation function, Alanyl-tRNA synthetase exhibits a remarkable editing domain, allowing it to rectify incorrectly charged tRNA(Ala). This dual functionality underscores the critical role of Alanyl-tRNA synthetase in maintaining the accuracy and fidelity of the translation process, emphasizing its significance in cellular protein synthesis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P49588 (M1-N968)

16  [NCBI]

Approximately 109.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 7.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.