ALCAM/CD166 Protein, Mouse (HEK293, Fc)

ALCAM/CD166 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD166 expressed by HEK293 cells with C-Fc tag. CD166, a member of the immunoglobulin superfamily, is widely expressed in various tissues, such as neurons, fibroblasts, endothelial cells, and keratinocytes^[1].

The CD166/activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and is widely expressed in various tissues, such as neurons, fibroblasts, endothelial cells, and keratinocytes. ALCAM is involved in neurogenesis, angiogenesis, hematopoiesis, leukocyte trafficking, and hematopoietic stem cell maintenance in bone marrow niches^[1].

Mouse

HEK293

C-Fc

Q61490 (W28-K527)

11658  [NCBI]

WYTVNSAYGDTIVMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSIANAKISDEKRFVCMLVTEDNVFEAPTLVKVFKQPSKPEIVNKAPFLETDQLKKLGDCISRDSYPDGNITWYRNGKVLQPVEGEVAILFKKEIDPGTQLYTVTSSLEYKTTRSDIQMPFTCSVTYYGPSGQKTIYSEQEIFDIYYPTEQVTIQVLPPKNAIKEGDNITLQCLGNGNPPPEEFMFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKRNMAASTTITVHYLDLSLNPSGEVTKQIGDTLPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIHWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADDISDENREKVNDQAK

110-120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Noriyuki Kijima, et al. CD166/Activated leukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion. Neuro Oncol. 2012 Oct; 14(10): 1254–1264.  [Content Brief]