ALDH4A1 Protein, Human (sf9)

The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free. The total length of ALDH4A1 Protein, Human (sf9) is 539 a.a., with molecular weight of ~54 KDa.

ALDH4A1, a pivotal enzyme, catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived from either proline or ornithine, into glutamate. This enzymatic transformation serves as a crucial step in the intricate pathway linking the urea and tricarboxylic acid cycles. The preferred substrate for ALDH4A1 is glutamic gamma-semialdehyde, while it also accepts other substrates such as succinic, glutaric, and adipic semialdehydes. The enzymatic activity of ALDH4A1 plays a central role in maintaining the metabolic balance between proline, ornithine, and glutamate, contributing to the efficient utilization of these amino acids within the interconnected cellular pathways.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

Tag Free

P30038 (K25-Q563)

8659  [NCBI]

Approximately 54 kDa.

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.