1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. Alkaline Phosphatase
  5. Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His)

Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700949
COA Handling Instructions

Alkaline Phosphatase (ALPP) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Zinc and magnesium are essential cofactors of ALPP. ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium. Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Alkaline Phosphatase/ALPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is 483 a.a., with molecular weight of 57-67 kDa.

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20 μg $160 In-stock
50 μg $305 In-stock
100 μg $490 In-stock
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  • Biological Activity

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Description

Alkaline Phosphatase (ALPP) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Zinc and magnesium are essential cofactors of ALPP. ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium[1][2][3]. Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Alkaline Phosphatase/ALPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is 483 a.a., with molecular weight of 57-67 kDa.

Background

Alkaline Phosphatase (ALPP), a group of isoenzymes, are usually located on the outer layer of the cell membrane. ALPP catalyzes the hydrolysis of organic phosphate esters found in the extracellular space. ALPP is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values[1]. Zinc and magnesium are essential cofactors of ALPP. Besides, ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium[3].
ALPs are categorized into 2 types: tissue-specific and tissue-nonspecific. Tissue-specific ALPs are exclusively present in the intestine, placenta, and germinal tissue. Under specific conditions, the tissue-specific ALPs may also contribute to the circulating pool of serum ALP. More than 80% of the ALP in serum originates from the liver and bone[2].

Biological Activity

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase, His Tag with an affinity constant of 79.52 nM as determined in SPR assay (Biacore T200).

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

XP_045223825.1 (I21-T503)

Gene ID
Molecular Construction
N-term
ALPP (I21-T503)
Accession # XP_045223825.1
His
C-term
Synonyms
Alkaline phosphatase; PLAP-1; ALPP; PLAP
Molecular Weight

57-67 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 25mM Tris, 100mM NaCl, 1mM MgCl2, pH 7.5.Normally 8%

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P700949
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