Alpha-Fetoprotein Protein, Human (HEK293, His)

Alpha-Fetoprotein, Human (HEK293, His) is a major plasma protein produced by the yolk sac and the fetal liver during fetal development.

Alpha-Fetoprotein is one of several tumor markers, such as hepatocellular carcinomas, cirrhosis, and chronic acute hepatitis. Tumor markers mean that molecules in the blood are higher when one has certain cancers^[1].
Alpha-Fetoprotein can be detected in liver cancer, nonseminomatous germ cell tumors, cirrhosis or chronic active hepatitis lso have higher blood levels of AFP^[1].

Human

HEK293

C-6*His

P02771 (T20-V609)

174  [NCBI]

TLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

approximately 60-70 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jing-Yue Yang, et al. Co-transfection of Dendritic Cells With AFP and IL-2 Genes Enhances the Induction of Tumor Antigen-Specific Antitumor Immunity. Exp Ther Med. 2012 Oct;4(4):655-660.  [Content Brief]

  [2]. Mingyue Zhu, et al. Hepatitis B Virus X Protein Induces Expression of Alpha-Fetoprotein and Activates PI3K/mTOR Signaling Pathway in Liver Cells.Oncotarget. 2015 May 20;6(14):12196-208.  [Content Brief]