AMIGO2 Protein, Human (HEK293, His)

AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane^[1].

AMIGO2 is a scaffold protein that regulates PDK1 membrane localization and Akt activation. This protein contains six leucine-rich repeats (LRRs), a single IgG-like domain, a transmembrane domain, and a cytosolic domain. AMIGO2 plays its role by controling PDK1 localization to the plasma membrane and then directly interacts with PDK1 to activate the Akt pathway^[1].
Loss of AMIGO2 in endothelial cells (ECs) leads to apoptosis and inhibition of angiogenesis with Akt inactivation^[1].
AMIGO2 is important in regulating T-cell functions, Amigo2-overexpression dampens NF-kB transcriptional activity, while Amigo2-deficiency enhances Akt but suppresses GSK-3β phosphorylation and promotes nuclear translocations of NF-kB and NFAT1 in Th-cells^[1].

Human

HEK293

C-6*His

Q86SJ2 (V40-A392)

347902  [NCBI]

VCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHHHHHHH

58-85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PB, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hyojin Park, et al.AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation. J Cell Biol  [Content Brief]

  [2]. Zhilin Li, et al. AMIGO2 modulates T cell functions and its deficiency in mice ameliorates experimental autoimmune encephalomyelitis. Brain Behav Immun. 2017 May;62:110-123.  [Content Brief]