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  5. AMIGO2 Protein, Mouse (HEK293, His)

AMIGO2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P77871
Handling Instructions

AMIGO2 Protein, crucial for the survival of cultured cerebellar granule neurons, mediates homophilic and heterophilic cell interactions with AMIGO1 or AMIGO3. Its intracellular domain may contribute to signal transduction, and AMIGO2 exhibits self-binding ability along with binding to AMIGO1 and AMIGO3 proteins. AMIGO2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived AMIGO2 protein, expressed by HEK293 , with C-His labeled tag. The total length of AMIGO2 Protein, Mouse (HEK293, His) is 359 a.a., with molecular weight of 55-65 kDa.

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Description

AMIGO2 Protein, crucial for the survival of cultured cerebellar granule neurons, mediates homophilic and heterophilic cell interactions with AMIGO1 or AMIGO3. Its intracellular domain may contribute to signal transduction, and AMIGO2 exhibits self-binding ability along with binding to AMIGO1 and AMIGO3 proteins. AMIGO2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived AMIGO2 protein, expressed by HEK293 , with C-His labeled tag. The total length of AMIGO2 Protein, Mouse (HEK293, His) is 359 a.a., with molecular weight of 55-65 kDa.
Background

AMIGO2 Protein is essential for the depolarization-dependent survival of cultured cerebellar granule neurons. It plays a crucial role in mediating both homophilic and heterophilic cell-cell interactions with AMIGO1 or AMIGO3. Additionally, it is speculated to contribute to signal transduction through its intracellular domain. Furthermore, AMIGO2 has the capability to bind to itself, as well as to AMIGO1 and AMIGO3 proteins.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

Q80ZD9 (M39-T397)
Gene ID
Molecular Construction
N-term
AMIGO2 (M39-T397)
Accession # Q80ZD9
His
C-term
Synonyms
Amphotein-induced protein 2; AMIGO-2; Alivin-1; DEGA; AMIGO2; ALI1; alivin 1
Molecular Weight

55-65 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

AMIGO2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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