Angiopoietin-2 Protein, Cynomolgus/Rhesus Macaque (HEK293, Fc)

Angiopoietin-2 (Ang2) is a growth factor that belongs to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, which is one of the main pathways involved in angiogenesis. Ang2 is a 496 amino acid long protein with a secretory signal peptide, an NH2-terminal coiled-coil domain, and a COOH-terminal fibrinogen-like domain. Ang2 mainly inhibits Ang1-induced Tie2 phosphorylation. Ang2 is critical for endothelial cell physiology and plays a central role in vascular-related diseases by regulating endothelial permeability and angiogenic functions. Angiopoietin-2 Protein, Cynomolgus/Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived Angiopoietin-2 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Angiopoietin-2 Protein, Cynomolgus/Rhesus Macaque (HEK293, Fc) is 222 a.a., with molecular weight of ~53.8 KDa.

Ang2, a 496 amino acid-long protein, shares ~60% amino acid homology with Ang1 and lacks one of the nine cysteines found in mature Ang1. It has a secretion signal peptide, an NH2-terminal coiled-coil domain, and a COOH-terminal fibrinogen-like domain. Unlike Ang1, Ang2 acts in an autocrine manner and its expression is highly regulated. Similar to Ang1, Ang2 binds to the Tie2 receptor with the same binding affinity, inducing its antagonistic role, but does not bind to Tie1. Ang2 expression is triggered by inflammatory mediators, such as thrombin [4], and conditions, such as hypoxia and cancer^[1].
ANGPT2 has been described as a context-dependent antagonist interfering with angiopoietin-1-induced Tie2 phosphorylation to destroy vascular stability and promote angiogenesis, might confer resistance to antiangiogenic therapy. ANGPT2 has been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation^[2].

Rhesus Macaque

HEK293

N-hFc

XP_005562586 (K274-F495)

101925495  [NCBI]

Approximately 53.8 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.