Animal-Free Activin A Protein, Human/Mouse/Rat

1.Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is ≤ 0.85 ng/mL. The specific activity of recombinant human Activin A is approximately >1.4 x 10³ IU/mg.
2.Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg.

Rat; Mouse; Human

E. coli

Tag Free

P08476 (G311-S426)

3624

MGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Approximately 13.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a solution of 0.1M Glycine, 150mM NaCl, pH 4.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.