Animal-Free BAFF/TNFSF13B Protein, Human (His)

BAFF/TNFSF13B protein is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway together with TNFSF13/APRIL. These interactions play a crucial role in stimulating B-cell and T-cell function and regulating humoral immunity. Animal-Free BAFF/TNFSF13B Protein, Human (His) is the recombinant human-derived animal-FreeBAFF/TNFSF13B protein, expressed by E. coli , with C-His labeled tag.

BAFF/TNFSF13B protein, a cytokine, binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway alongside TNFSF13/APRIL. Together, these interactions play a crucial role in stimulating B- and T-cell function and regulating humoral immunity. Notably, a third B-cell-specific receptor, BAFFR/BR3, is involved in promoting the survival of mature B-cells and facilitating the B-cell response. This intricate network underscores the significance of BAFF/TNFSF13B in orchestrating immune responses. Additionally, isoform 2 of BAFF/TNFSF13B appears to exert a regulatory role by inhibiting the secretion and bioactivity of isoform 1. The dynamic interplay between these isoforms further contributes to the nuanced control of BAFF/TNFSF13B-mediated immune processes.

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.5 ng/mL.

Human

E. coli

C-His

Q9Y275-1 (A134-L285)

10673  [NCBI]

MAVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Approximately 17.98 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.