2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His)

Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His)

Cat. No.: HY-P701239AF
COA Handling Instructions

Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His) is the recombinant SUMO Protease, expressed by E. coli , with His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
1000 U $495 In-stock
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His) is the recombinant SUMO Protease, expressed by E. coli , with His labeled tag. ,
Biological Activity

10 U/μl .
Unit definition: One unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate in 1 h at 30°C.
Species

Others
Source

E. coli
Tag

His
Accession

/
Gene ID

/
Synonyms
SUMO protease
AA Sequence

/

Molecular Weight

N/A
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 0.1% Igepal (NP-40), 250 mM NaCl, 0.5 mM DTT, 50% (v/v) glycerol, pH 8.0.
Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method
Reconstitution

N/A.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Shipping with dry ice.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Animal-Free SUMO Protease Protein, Saccharomyces cerevisiae (His)
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