Annexin A1/ANXA1 Protein, Human

Annexin A1 Protein, Human is an approximately 34.0 kDa Annexin A1 protein expressed by the E. coli system. Annexin A1 is a Ca²⁺-dependent phospholipid binding protein and has inhibitory effects on phospholipase A₂ (PLA₂)^[1].

Annexin A1 is a member of the Ca²⁺-dependent phospholipid binding proteins that are localized on the cytosolic face of the plasma membrane. Annexin A1 inhibits activity of phospholipase A2, binding 2-4 calcium ions with high affinity. Annexin A1 is a protein with multifunctional roles in innate and adaptive immunity mainly devoted to the regulation of inflammatory cells and the resolution of inflammation. Annexin A1 is an anti-inflammatory molecule playing major role as an inhibitor of neutrophil transmigration and recruitment at inflammatory sites^[1][2].

Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED₅₀ for this effect is ≤2 μg/mL, corresponding to a specific activity is ≥500 units/mg.

• 
  Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED₅₀ for this effect is 0.7223 μg/mL, corresponding to a specific activity is 1.38×10³ units/mg.

Human

E. coli

Tag Free

P04083 (A2-N346)

301  [NCBI]

AMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

34-39 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zied Boudhraa,et al. Annexin A1 localization and its relevance to cancer. Clin Sci (Lond). 2016 Feb;130(4):205-20.  [Content Brief]

  [2]. Maurizio Bruschi, et al. Annexin A1 and Autoimmunity: From Basic Science to Clinical Applications. Int J Mol Sci. 2018 May 3;19(5):1348.  [Content Brief]