Annexin A2/ANXA2 Protein, Human

Annexin A2/ANXA2 Protein, Human is a 38kDa multifunctional protein which belongs to the annexin family. Annexin A2 is important for the regulation in the cellular growth and in signal transduction pathways. Annexin A2 also can be used as an autocrine factor that can heighten osteoclast formation and bone resorption^[1].

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin A2 is an important member of annexin family of proteins expressed on surface of endothelial cells (ECs), macrophages, mononuclear cells and various types of cancer cells. Annexin A2 exhibits high affinity binding for calcium (Ca²⁺) and phospholipids. ANX A2 plays an important role in many biological processes such as endocytosis, exocytosis, autophagy, cell-cell communications and biochemical activation of plasminogen.

Measured in a cell proliferation assay using A549 cells. The ED₅₀ this effect is ≤1.051 ng/mL, corresponding to a specific activity is ≥9.515×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The The ED₅₀ for this effect is 0.7184 ng/mL, corresponding to a specific activity is 1.39×10⁶ units/mg.

Human

E. coli

Tag Free

P07355-1 (S2-D339)

302  [NCBI]

STVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Approximately 38 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM Histidine-HCl, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 5.5. or PBS, pH 7.4 or PBS, pH 7.4, 6% saccharose, 4% mannitol and 0.05% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Mahesh C Sharma, et al. Annexin A2 (ANX A2): An emerging biomarker and potential therapeutic target for aggressive cancers. Int J Cancer. 2019 May 1;144(9):2074-2081.  [Content Brief]