Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His)

Antithrombin III, also known as Serpin C1, forms a protease inhibitory heterodimer with TMPRSS7. This interaction exemplifies the regulatory role of antithrombin III as a serine protease inhibitor, highlighting its ability to modulate TMPRSS7 activity. Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Antithrombin III/Serpin C1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is 430 a.a., with molecular weight of ~55-75 kDa.

Antithrombin III (ATIII), also known as Serpin C1, is a crucial serine protease inhibitor that forms a protease inhibiting heterodimer with TMPRSS7. This interaction underlines ATIII's role in regulating coagulation processes by inhibiting the activity of various proteases involved in the blood clotting cascade. The formation of a heterodimer with TMPRSS7 emphasizes the specificity of ATIII in targeting and modulating the activity of specific proteases. This protease inhibiting function plays a central role in preventing excessive blood clotting and maintaining hemostatic balance.

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC₅₀ value is 2.185 nM, as measured under the described conditions.

Cynomolgus

HEK293

C-His

A0A2K5UYN7 (S35-S464)

102117641  [NCBI]

SPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDKDNIFLSPLSVSTAFAMTKLGACNDTLKQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPPEAINELTVLVLVNTIYFKGLWKSKFSPENTRMEPFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLTKVEQELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAIGIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVS

Approximately 55-75 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.