APAF1 Protein, Human (P. pastoris, His)

APAF1 Protein, a pivotal apoptosome component, orchestrates cytochrome c-dependent activation of pro-caspase-9, initiating caspase-3 activation and apoptosis. Forming an apoptosome ring with cytochrome c and dATP, APAF1 interacts with pro-caspase-9, releasing mature caspase-9. Pro-caspase-3 joins the complex, activating caspase-3. Notably, APAF1 isoform 6 is less effective in inducing apoptosis. APAF1 interacts with APIP, NAIP/BIRC1, and CIAO2A, contributing to its multifaceted role in apoptotic regulation. APAF1 Protein, Human (P. pastoris, His) is the recombinant human-derived APAF1 protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of APAF1 Protein, Human (P. pastoris, His) is 312 a.a., with molecular weight of 37.4 kDa.

APAF1 Protein, as an essential component of the apoptosome, orchestrates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), initiating the caspase-3 activation cascade and ultimately leading to apoptosis. The oligomeric form of APAF1 forms a heptameric ring, known as the apoptosome, in the presence of cytochrome c and dATP. Within this complex, APAF1 and pro-caspase-9 interact via their NH2-terminal CARD domains, leading to the release of mature caspase-9. Concurrently, pro-caspase-3 is recruited into the APAF1-pro-caspase-9 complex through interaction with pro-caspase-9, facilitating the activation of caspase-3. Notably, isoform 6 of APAF1 exhibits reduced effectiveness in inducing apoptosis. Additionally, APAF1 interacts with APIP, NAIP/BIRC1, and CIAO2A, contributing to its multifaceted role in the regulation of apoptotic pathways.

Human

P. pastoris

N-6*His

O14727 (S104-L415)

317  [NCBI]

SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL

37.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.