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  4. Apolipoprotein L/APOL1 Protein, Human (sf9, His)

Apolipoprotein L/APOL1 Protein, Human (sf9, His)

Cat. No.: HY-P75504
Handling Instructions

Apolipoprotein L (APOL1) Protein is a member of the apolipoprotein L family. Apolipoprotein L/APOL1 Protein, Human (sf9, His) is the recombinant human-derived Apolipoprotein L/APOL1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Apolipoprotein L/APOL1 Protein, Human (sf9, His) is 371 a.a., with molecular weight of ~44 kDa.

For research use only. We do not sell to patients.

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Description

Apolipoprotein L (APOL1) Protein is a member of the apolipoprotein L family. Apolipoprotein L/APOL1 Protein, Human (sf9, His) is the recombinant human-derived Apolipoprotein L/APOL1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Apolipoprotein L/APOL1 Protein, Human (sf9, His) is 371 a.a., with molecular weight of ~44 kDa.
Background

Apolipoprotein L/APOL1 Protein is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.
Species

Human
Source

Sf9 insect cells
Tag

C-His
Accession

Q2KHQ6 (E28-L398)
Gene ID
Molecular Construction
N-term
APOL1 (E28-L398)
Accession # Q2KHQ6
His
C-term
Synonyms
Apolipoprotein L1; Apo-L; ApoL-I; APOL1; APOL
Molecular Weight

Approximately 44 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Apolipoprotein L/APOL1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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