ASAH1 Protein, Human (His-SUMO)

ASAH1 is a lysosomal ceramidase that critically regulates sphingolipid metabolism by hydrolyzing ceramide into sphingosine and fatty acids at acidic pH. Bioactive lipids—ceramide, sphingosine, and sphingosine-1-phosphate—control signaling pathways for cell proliferation, apoptosis, and differentiation. ASAH1 Protein, Human (His-SUMO) is the recombinant human-derived ASAH1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of ASAH1 Protein, Human (His-SUMO) is 374 a.a., with molecular weight of ~58.7 kDa.

ASAH1, a lysosomal ceramidase, functions in sphingolipid metabolism by hydrolyzing sphingolipid ceramides into sphingosine and free fatty acids under acidic pH conditions. Ceramides, sphingosine, and sphingosine-1-phosphate are bioactive lipids that play crucial roles in cellular signaling pathways governing processes such as cell proliferation, apoptosis, and differentiation. ASAH1 exhibits higher catalytic efficiency towards C12-ceramides compared to other ceramides and can also catalyze the reverse reaction, synthesizing ceramides from fatty acids and sphingosine. In this synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized, with fatty acids of chain lengths 12 or 14 carbons being the most effective substrates. Additionally, ASAH1 displays N-acylethanolamine hydrolase activity. By regulating ceramide, sphingosine, and sphingosine-1-phosphate levels, ASAH1 mediates calcium-induced differentiation of epidermal keratinocytes. It indirectly regulates tumor necrosis factor (TNF)-induced apoptosis and, in adrenocortical cells, likely serves as a regulator of steroidogenesis by maintaining the intracellular balance between ceramides and sphingosine. Furthermore, ASAH1 may directly impact steroidogenesis by binding to the nuclear receptor NR5A1 and negatively regulating its transcriptional activity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q13510 (22Q-395W)

427  [NCBI]

QHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Approximately 58.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.