ASGR2/ASGPR2 Protein, Human (HEK293, His)

ASGR2 mediates endocytosis of desialylated glycoproteins, recognizing galactose and N-acetylgalactosamine units. Ligand binding leads to internalization and sorting organelle transport, followed by receptor return to the cell membrane. The receptor is suggested to function as a dimer. ASGR2 also interacts with LASS2. ASGR2/ASGPR2 Protein, Human (HEK293, His) is the recombinant human-derived ASGR2 protein, expressed by HEK293 , with N-His labeled tag.

The ASGR2 protein serves as a mediator in the endocytosis of plasma glycoproteins, particularly those from which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. This receptor exhibits recognition capabilities for terminal galactose and N-acetylgalactosamine units. Upon ligand binding to the receptor, the resulting complex undergoes internalization and is transported to a sorting organelle, where receptor and ligand disassociate. Subsequently, the receptor returns to the cell membrane surface. It is suggested that the functioning ligand-binding unit of this receptor is at least a dimer. Additionally, ASGR2 engages in interactions with LASS2.

Human

HEK293

N-His

P07307 (Q80-A311)

433  [NCBI]

37-42 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.