1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. ASPH Protein, Mouse (His)

ASPH Protein, Mouse (His)

Cat. No.: HY-P77565
Handling Instructions

ASPH proteins exhibit enzymatic specificity by selectively hydroxylating aspartate (Asp) or asparagine (Asn) residues within specific epidermal growth factor-like (EGF) domains of the protein. This site-specific hydroxylation regulates post-translational modifications of EGF-containing proteins, potentially affecting their conformation, stability, or interactions. ASPH Protein, Mouse (His) is the recombinant mouse-derived ASPH protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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  • Documentation

Description

ASPH proteins exhibit enzymatic specificity by selectively hydroxylating aspartate (Asp) or asparagine (Asn) residues within specific epidermal growth factor-like (EGF) domains of the protein. This site-specific hydroxylation regulates post-translational modifications of EGF-containing proteins, potentially affecting their conformation, stability, or interactions. ASPH Protein, Mouse (His) is the recombinant mouse-derived ASPH protein, expressed by E. coli , with N-His labeled tag.

Background

ASPH protein demonstrates specificity in its enzymatic function, as it selectively hydroxylates an aspartic acid (Asp) or asparagine (Asn) residue within specific epidermal growth factor-like (EGF) domains of various proteins. This site-specific hydroxylation activity contributes to the post-translational modification of proteins containing EGF-like domains, potentially influencing their structural conformation, stability, or interaction capabilities. ASPH's specificity in targeting Asp or Asn residues within these domains underscores its role as a key regulator in modulating the functional properties of EGF-containing proteins, highlighting its significance in cellular signaling pathways and molecular processes influenced by EGF-like domains.

Species

Mouse

Source

E. coli

Tag

N-His

Accession

Q8BSY0-1 (I324-I741)

Gene ID
Molecular Construction
N-term
His
ASPH (I324-I741)
Accession # Q8BSY0-1
C-term
Synonyms
Aspartate beta-hydroxylase; ASP beta-hydroxylase; Asph; Bah
Molecular Weight

Approximately 49.4 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ASPH Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ASPH Protein, Mouse (His)
Cat. No.:
HY-P77565
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