ASS1 Protein, Human (His)

ASS1 Protein, Human (His) is a recombinant human Argininosuccinate synthase expressed in E. coli with a His tag. Argininosuccinate synthase is an enzyme that catalyzes the synthesis of argininosuccinate from citrulline and aspartate^[1].

Arginine is required by all tissues in the human body for protein synthesis, and by some tissues for specialized needs. Any de novo biosynthetic pathway for arginine involves the conversion of citrulline to arginine catalyzed by argininosuccinate synthase and argininosuccinate lyase. Specifically, argininosuccinate synthase catalyzes the condensation of citrulline and aspartate to form argininosuccinate, the immediate precursor of arginine^[1].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P00966 (M1-K412)

445  [NCBI]

HHHHHHMSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, 50 mM Imidazole, 1 mM DTT, 40% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Ricci J Haines, et al. Argininosuccinate synthase: at the center of arginine metabolism. Int J Biochem Mol Biol. 2011;2(1):8-23.  [Content Brief]