Ataxin-3 Protein, Human (His)

Ataxin-3 is a deubiquitinating enzyme that maintains proteostasis, regulates transcription, and is involved in cytoskeletal dynamics, myogenesis, and degradation of misfolded chaperone substrates. It trims long polyubiquitin chains and interacts with STUB1/CHIP to limit ubiquitin chain length, preventing further elongation. Ataxin-3 Protein, Human (His) is the recombinant human-derived Ataxin-3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Ataxin-3 Protein, Human (His) is 361 a.a., with molecular weight of ~43 kDa.

Ataxin-3 protein, a deubiquitinating enzyme, plays a crucial role in maintaining protein homeostasis, regulating transcription, cytoskeleton dynamics, myogenesis, and participating in the degradation of misfolded chaperone substrates. Demonstrating an affinity for long polyubiquitin chains, Ataxin-3 trims them, particularly focusing on chains with more ubiquitin moieties, while exhibiting weak or no activity against shorter chains. It engages in the degradation of misfolded chaperone substrates by interacting with STUB1/CHIP, where it restricts the length of ubiquitin chains attached to STUB1/CHIP substrates, preventing further chain extension. Additionally, Ataxin-3 acts as a histone-binding protein, influencing transcription regulation, and serves as a negative regulator of mTORC1 signaling during amino acid deprivation by mediating the deubiquitination of RHEB. Furthermore, Ataxin-3 contributes to autophagy regulation by deubiquitinating 'Lys-402' of BECN1, leading to BECN1 stabilization.

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ this effect is 2.979 ng/ml, corresponding to a specific activity is 3.36×10⁵ units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ for this effect is 2.979 ng/ml, corresponding to a specific activity is 3.36×10⁵ units/mg.

Human

E. coli

N-6*His

P54252-2 (M1-K361)

4287  [NCBI]

MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK

Approximately 43 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.