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Bcl-XL Protein, Mouse (C-His)

Cat. No.: HY-P72846A
SDS COA Handling Instructions

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (C-His) is the recombinant mouse-derived Bcl-XL, expressed by E. coli , with C-6*His labeled tag. The total length of Bcl-XL Protein, Mouse (C-His) is 212 a.a.,

For research use only. We do not sell to patients.

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10 μg $78 In-stock
50 μg $220 In-stock
100 μg $350 In-stock
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  • Biological Activity

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Description

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (C-His) is the recombinant mouse-derived Bcl-XL, expressed by E. coli , with C-6*His labeled tag. The total length of Bcl-XL Protein, Mouse (C-His) is 212 a.a.,

Background

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, with heterodimerization with BAX not being essential for its anti-apoptotic activity. Additionally, it interacts with isoform 1 of SIVA1, inhibiting its anti-apoptotic function. The protein also engages with IKZF3 and RTL10/BOP. Furthermore, interactions with DNM1L and CLTA suggest the potential formation of a complex in synaptic vesicles that includes clathrin and MFF. Notably, Bcl-XL interacts with NLRP1, specifically via the loop between motifs BH4 and BH3, but does not engage with NLRP2, NLRP3, NLRP4, PYCARD, or MEFV. It also interacts with BECN1, indicating its involvement in autophagy regulation.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human BID at 10 μg/mL can bind biotinylated mouse BCL2L1. The ED50 for this effect is 5.195 ng/mL.

Species

Mouse

Source

E. coli

Tag

C-6*His

Accession

Q64373-1 (M1-R212)

Gene ID

12048

Synonyms
Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; Bclx
AA Sequence

MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNR

Molecular Weight

Approximately 27 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose, 0.02% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Bcl-XL Protein, Mouse (C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Bcl-XL Protein, Mouse (C-His)
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HY-P72846A
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