beta-Catenin Protein, Mouse (sf9, His-GST)

β-Catenin is a key component of canonical Wnt signaling, which dynamically controls cellular processes. In the absence of Wnt, it forms a complex leading to phosphorylation and degradation. beta-Catenin Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of beta-Catenin Protein, Mouse (sf9, His-GST) is 781 a.a., with molecular weight of ~115 kDa.

Beta-catenin, a pivotal player in the canonical Wnt signaling pathway, dynamically regulates cellular processes in response to Wnt ligands. In the absence of Wnt, beta-catenin participates in a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting its phosphorylation and ubiquitination, leading to subsequent degradation by the proteasome. Upon Wnt activation, beta-catenin accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family, thereby activating Wnt-responsive genes. This multifaceted protein also plays a role in cell adhesion, acting as a component of an E-cadherin:catenin adhesion complex. Beyond these functions, beta-catenin emerges as a negative regulator of centrosome cohesion and exhibits roles in insulin internalization, inhibition of anoikis, and promotion of neurogenesis. It modulates chondrocyte differentiation, positively regulates odontoblast differentiation during tooth germ formation, and interacts with various proteins, including regulators and effectors of Wnt signaling, highlighting its central role in diverse cellular processes. Beta-catenin's complex network of interactions underscores its significance in orchestrating cellular responses to intricate signaling cues, contributing to the regulation of critical cellular functions.

Mouse

Sf9 insect cells

N-His;N-GST

Q02248 (M1-L781)

12387  [NCBI]

Approximately 115 kDa

Greater than 88% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, 1 mM TCEP, 0.1 mM EDTA, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.