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  3. beta-Catenin Protein, Mouse (sf9, His-GST)

β-Catenin is a key component of canonical Wnt signaling, which dynamically controls cellular processes. In the absence of Wnt, it forms a complex leading to phosphorylation and degradation. beta-Catenin Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of beta-Catenin Protein, Mouse (sf9, His-GST) is 781 a.a., with molecular weight of ~115 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg USD 89 In-stock
20 μg USD 147 In-stock
50 μg USD 278 In-stock
100 μg USD 446 In-stock
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beta-Catenin Protein, Mouse (sf9, His-GST) Featured Recommendations:

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Description

β-Catenin is a key component of canonical Wnt signaling, which dynamically controls cellular processes. In the absence of Wnt, it forms a complex leading to phosphorylation and degradation. beta-Catenin Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of beta-Catenin Protein, Mouse (sf9, His-GST) is 781 a.a., with molecular weight of ~115 kDa.

Background

Beta-catenin, a pivotal player in the canonical Wnt signaling pathway, dynamically regulates cellular processes in response to Wnt ligands. In the absence of Wnt, beta-catenin participates in a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting its phosphorylation and ubiquitination, leading to subsequent degradation by the proteasome. Upon Wnt activation, beta-catenin accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family, thereby activating Wnt-responsive genes. This multifaceted protein also plays a role in cell adhesion, acting as a component of an E-cadherin:catenin adhesion complex. Beyond these functions, beta-catenin emerges as a negative regulator of centrosome cohesion and exhibits roles in insulin internalization, inhibition of anoikis, and promotion of neurogenesis. It modulates chondrocyte differentiation, positively regulates odontoblast differentiation during tooth germ formation, and interacts with various proteins, including regulators and effectors of Wnt signaling, highlighting its central role in diverse cellular processes. Beta-catenin's complex network of interactions underscores its significance in orchestrating cellular responses to intricate signaling cues, contributing to the regulation of critical cellular functions.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q02248 (M1-L781)

Gene ID
Molecular Construction
N-term
His-GST
beta-Catenin (M1-L781)
Accession # Q02248
C-term
Synonyms
beta-Catenin; Catenin beta-1; CTNNB; CTNNB1
Molecular Weight

Approximately 115 kDa

Purity

Greater than 88% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, 1 mM TCEP, 0.1 mM EDTA, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

beta-Catenin Protein, Mouse (sf9, His-GST) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
beta-Catenin Protein, Mouse (sf9, His-GST)
Cat. No.:
HY-P74381
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