Beta-galactosidase/GLB1 Protein, Human (HEK293, His)

The beta-galactosidase/GLB1 protein cleaves beta-linked terminal galactose residues in gangliosides and glycoproteins, contributing to elastogenesis and connective tissue development. Beta-galactosidase/GLB1 Protein, Human (HEK293, His) is the recombinant human-derived Beta-galactosidase/GLB1 protein, expressed by HEK293 , with C-6*His labeled tag.

The Beta-galactosidase (GLB1) protein functions as an enzyme that cleaves beta-linked terminal galactosyl residues from various substrates, including gangliosides, glycoproteins, and glycosaminoglycans. Despite lacking direct beta-galactosidase catalytic activity, GLB1 plays essential functional roles in elastogenesis, contributing to the formation of extracellular elastic fibers and the development of connective tissue. Intriguingly, GLB1 is identified as the elastin-binding protein (EBP), a crucial component of the non-integrin cell surface receptor found on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin-producing cells, GLB1 associates with tropoelastin intracellularly, serving as a recycling molecular chaperone that facilitates the secretion of tropoelastin and supports its assembly into elastic fibers. This multifaceted functionality positions GLB1 as a key player in both enzymatic processes and structural roles related to tissue development and maintenance.

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta-D-galactopyranoside. The specific activity is 1151.98 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

P16278-1 (L24-V677)

2720  [NCBI]

LRNATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

Approximately 90.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.