BirA Protein, E. coli (His-MBP)

BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli , with N-His, N-MBP labeled tag.

WBP2 protein functions as a transcriptional coactivator for estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation, binding to ESR1-responsive promoters in the presence of estrogen. It is essential for the coactivation function of YAP1 on PGR activity and synergizes with WBP2 in enhancing PGR activity. Additionally, WBP2 modulates the expression of post-synaptic scaffolding proteins through the regulation of ESR1, ESR2, and PGR. WBP2 interacts with the WW domain of YAP1, WWP1, and WWP2, and also forms interactions with NEDD4. Moreover, it associates with ESR1 and UBE3A, contributing to its role as a transcriptional coactivator in hormone-responsive pathways.

Measured by its ability to generate pyrophosphate from the biotinylation reaction. The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (HY-P79310) that incubated at room temperature for 15 min. The specific activity is 654.92 pmol/min/μg.

E.coli

E. coli

N-His;N-MBP

P06709 (M1-K321)

948469  [NCBI]

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK

Approximately 64-77 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0 or PBS, pH 7.4, 8% trehalose, 8% mannitol and 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.