BIRC5 Protein, Human (His-SUMO)

The BIRC5 protein has dual roles in cell proliferation and prevention of apoptosis. It is an important CPC component and guides chromosome alignment and segregation during mitosis. It directs CPC movement, contributes to central spindle organization, and recruits CPC to centromeres. BIRC5 Protein, Human (His-SUMO) is the recombinant human-derived BIRC5 protein, expressed by E. coli , with labeled tag.

BIRC5, a versatile protein, plays dual roles in promoting cell proliferation and preventing apoptosis, highlighting its multifaceted functions. As a crucial component of the chromosome passage protein complex (CPC), BIRC5 is integral to chromosome alignment and segregation during mitosis and cytokinesis. It orchestrates CPC movement, directing its localization from the inner centromere in prometaphase to the midbody during cytokinesis, and contributes to central spindle organization by associating with polymerized microtubules. BIRC5 is intricately involved in recruiting CPC to centromeres during early mitosis, binding to histone H3 phosphorylated at 'Thr-3' (H3pT3). Functioning in collaboration with RAN, it aids in mitotic spindle formation, serving as a scaffold for delivering the RAN effector molecule TPX2 to microtubules. Beyond its mitotic functions, BIRC5 counters default apoptosis induction in G2/M phase, and its acetylated form represses STAT3 transactivation of target gene promoters. Additionally, it serves as an inhibitor of CASP3 and CASP7, essential for maintaining mitochondrial integrity and function. Existing as a monomer in the CPC-bound state, BIRC5 efficiently protects cells against apoptosis, while its dimeric form enhances tubulin stability. BIRC5 engages in a complex network of interactions with various proteins, such as histone H3, RAN, tubulin, XIAP/BIRC4, and DIABLO/SMAC, underscoring its pivotal regulatory functions in cellular processes.

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 0.022 μg/mL, corresponding to a specific activity is 4.545×10⁴ units/mg.

Human

E. coli

N-6*His;N-SUMO

O15392-1 (M1-D142)

332

MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.