BLVRB Protein, Human (His)

The BLVRB protein is a broad-specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of substrates, including flavin, biliverdin, methemoglobin, and PQQ. It is essential in heme catabolism, metabolizing linear tetrapyrrole and reducing complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. BLVRB Protein, Human (His) is the recombinant human-derived BLVRB protein, expressed by E. coli , with N-His labeled tag. The total length of BLVRB Protein, Human (His) is 205 a.a., with molecular weight of ~25 KDa.

BLVRB Protein is a broad-specificity oxidoreductase with the ability to catalyze the NADPH-dependent reduction of various flavins, including riboflavin, FAD or FMN, as well as biliverdins, methemoglobin, and PQQ (pyrroloquinoline quinone). This enzyme plays a crucial role in heme catabolism, metabolizing linear tetrapyrroles. Additionally, it can reduce complexed Fe(3+) iron to Fe(2+) when FMN and NADPH are present. In the liver, BLVRB Protein serves a significant function by converting biliverdin to bilirubin, contributing to essential metabolic processes and maintaining redox balance.

Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is 733.394 pmoL/min/μg, as measured under the described conditions.

Human

E. coli

N-His

P30043 (A2-Q206)

645  [NCBI]

AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ

Approximately 25 kDa.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.