BPTF Protein, Human (His)

BPTF proteins are regulatory subunits in the NURF-1 and NURF-5 ISWI chromatin remodeling complexes that assemble ordered nucleosome arrays to access DNA during replication, transcription, and repair. The NURF-1 complex is critical for brain development and actively regulates the expression of En1 and En2. BPTF Protein, Human (His) is the recombinant human-derived BPTF protein, expressed by E. coli , with N-6*His labeled tag. The total length of BPTF Protein, Human (His) is 169 a.a., .

BPTF protein functions as a regulatory subunit within the ATP-dependent NURF-1 and NURF-5 ISWI chromatin remodeling complexes, orchestrating the assembly of ordered nucleosome arrays on chromatin to facilitate DNA access during essential processes such as DNA replication, transcription, and repair. The NURF-1 ISWI chromatin remodeling complex, characterized by a lower ATP hydrolysis rate compared to NURF-5, plays a pivotal role in brain development by binding to the promoters of En1 and En2, positively regulating their expression. BPTF, as a histone-binding protein, exhibits a preference for H3 tails trimethylated on 'Lys-4' (H3K4me3), a marker for transcription start sites of active genes, and interacts with dimethylated H3 tails to a lesser extent. Beyond histone binding, BPTF may directly regulate transcription through interaction with DNA or transcription factors. It interacts with MAZ and KEAP1 and is a crucial component of the NURF-1 ISWI chromatin remodeling complex, which includes SMARCA1, BPTF, RBBP4, and RBBP7. Additionally, BPTF forms the catalytically inactive BPFT-SMARCA1 complex and is a component of the NURF-5 ISWI chromatin remodeling complex with SMARCA5/SNF2H. These interactions underscore the multifaceted role of BPTF in chromatin dynamics and transcriptional regulation.

Human

E. coli

N-6*His

Q12830 (D2865-A3033)

2186

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.