2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. BRAF Protein, Human (Flag)

BRAF Protein, Human (Flag)

Cat. No.: HY-P703549
Handling Instructions

BRAF Protein, Human (Flag) is the recombinant human-derived BRAF, expressed by E. coli , with Flag labeled tag. The total length of BRAF Protein, Human (Flag) is 75 a.a.,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

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Description

BRAF Protein, Human (Flag) is the recombinant human-derived BRAF, expressed by E. coli , with Flag labeled tag. The total length of BRAF Protein, Human (Flag) is 75 a.a.,
Species

Human
Source

E. coli
Tag

Flag
Accession

P15056 (P155-N229)
Gene ID

673
Synonyms
BRAF1; RAFB1
Molecular Weight

Approximately 9.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

BRAF Protein, Human (Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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