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  4. BRAF Protein, Human (sf9, GST, His)

BRAF Protein, Human (sf9, GST, His)

Cat. No.: HY-P702921
SDS COA Handling Instructions

BRAF Protein, Human (sf9, GST, His) is the recombinant human-derived BRAF, expressed by Sf9 insect cells .

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg $1080 In-stock
50 μg   Get quote  
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

BRAF Protein, Human (sf9, GST, His) is the recombinant human-derived BRAF, expressed by Sf9 insect cells .

Biological Activity

The BRAF activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25°C for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.

Species

Human

Source

Sf9 insect cells

Tag

C-6*His;N-GST

Accession

P15056 (E433-R726)

Gene ID

673

Molecular Construction
N-term
GST
BRAF (E433-R726)
Accession # P15056
C-term
Synonyms
BRAF1; RAFB; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight

Approximately 61.3 kDa

Purity

Greater than 80% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

BRAF Protein, Human (sf9, GST, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
BRAF Protein, Human (sf9, GST, His)
Cat. No.:
HY-P702921
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