2. Recombinant Proteins
  3. Immune Checkpoint Proteins
  4. Butyrophilins
  5. BTN3A3
  6. BTN3A3 Protein, Human (HEK293, His)

BTN3A3 Protein, Human (HEK293, His)

Cat. No.: HY-P7685
Handling Instructions

BTN3A3 Protein, Human (HEK293, His) is a recombinant human BTN3A3 produced in HEK293 cells, with His tag. BTN3A3 is a member of butyrophilin family.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

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Description

BTN3A3 Protein, Human (HEK293, His) is a recombinant human BTN3A3 produced in HEK293 cells, with His tag. BTN3A3 is a member of butyrophilin family[1].
Background

Butyrophilins belonging to the immunoglobulin superfamily are new immune system regulators because they are present on lymphocytes, dendritic cells, monocytes, macrophages, neutrophils and eosinophils, and they exert a stimulatory and (or) inhibitory effect on them[1].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

O00478 (Q30-W248)
Gene ID
Molecular Construction
N-term
BTN3A3 (Q30-W248)
Accession # O00478
6*His
C-term
Synonyms
rHuBTN3A3, His; Butyrophilin subfamily 3 member A3; BTN3A3
AA Sequence

QFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELRWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHIEVKGYEDGGIHLECRSTGWYPQPQIKWSDTKGENIPAVEAPVVADGVGLYAVAASVIMRGSSGGGVSCIIRNSLLGLEKTASISIADPFFRSAQPWHHHHHH

Molecular Weight

Approximately 30.0 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
References

BTN3A3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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