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  4. Transferases (EC 2)
  5. BUB1-BUB3 Protein, Human (sf9, GST, Flag)

BUB1-BUB3 Protein, Human (sf9, GST, Flag)

Cat. No.: HY-P702928
Handling Instructions

BUB1-BUB3 Protein, Human (sf9, GST, Flag) is the recombinant human-derived BUB1-BUB3, expressed by Sf9 insect cells , with N-GST, C-Flag labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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50 μg   Get quote  
100 μg   Get quote  

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

BUB1-BUB3 Protein, Human (sf9, GST, Flag) is the recombinant human-derived BUB1-BUB3, expressed by Sf9 insect cells , with N-GST, C-Flag labeled tag. ,
Species

Human
Source

Sf9 insect cells
Tag

N-GST;C-Flag
Accession

O43683-1 (M1-K1085)&O43684-1 (M1-T328)
Gene ID

699
Synonyms
BUB1L; BUB1A; hBUB1; BUB3
Molecular Weight

BUB1: 149.3 kDa; BUB3: 38.4 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

BUB1-BUB3 Protein, Human (sf9, GST, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BUB1-BUB3 Protein, Human (sf9, GST, Flag)
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