1. Recombinant Proteins
  2. Complement System
  3. Complement Component 2
  4. C2/Complement C2 Protein, Mouse (HEK293, His)

C2/Complement C2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P74374
COA Handling Instructions

Complement C2 is a component of the classical pathway of the complement system and is cleaved by activated factor C1, resulting in the formation of two distinct fragments: C2b and C2a. The subsequent serine protease activity of C2a is critical for its subsequent interaction with the complement factor C4b, leading to the formation of C3 or C5 convertase. C2/Complement C2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $55 In-stock
10 μg $85 In-stock
50 μg $240 In-stock
100 μg $385 In-stock
500 μg $1080 In-stock
1 mg $1720 In-stock
> 1 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Complement C2 is a component of the classical pathway of the complement system and is cleaved by activated factor C1, resulting in the formation of two distinct fragments: C2b and C2a. The subsequent serine protease activity of C2a is critical for its subsequent interaction with the complement factor C4b, leading to the formation of C3 or C5 convertase. C2/Complement C2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag.

Background

Complement C2, a vital component of the classical pathway within the complement system, undergoes cleavage by activated factor C1 into two fragments: C2b and C2a. The generated C2a fragment, possessing serine protease activity, subsequently associates with complement factor C4b, culminating in the formation of the C3 or C5 convertase. This enzymatic complex plays a pivotal role in the activation cascade of the complement system, facilitating the downstream proteolytic cleavage of complement factors C3 and C5, which are integral steps in the immune response. The orchestrated interplay of C2 in the classical pathway underscores its significance in mediating immune defense mechanisms and maintaining immune homeostasis. (

Biological Activity

Measured by its ability to cleave a colorimetric peptide 200 μM substrate Z-GR-SBzl, in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 129.61 pmol/min/μg.

Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

P21180-1 (A19-L760)

Gene ID
Molecular Construction
N-term
C2 (M1-L760)
Accession # P21180
His
C-term
Synonyms
Complement C2; C3/C5 convertase; C2
AA Sequence

ALFCNQNVNITGGNFTLSHGWAPGSLLIYSCPLGRYPSPAWRKCQSNGQWLTPRSSSHHTLRSSRMVKAVCKPVRCLAPSSFENGIYFPRLVSYPVGSNVSFECEQDFTLRGSPVRYCRPNGLWDGETAVCDNGASHCPNPGISVGTARTGLNFDLGDKVRYRCSSSNMVLTGSAERECQSNGVWSGSEPICRQPYSYDFPEDVASALDTSLTNLLGATNPTQNLLTKSLGRKIIIQRSGHLNLYLLLDASQSVTEKDFDIFKKSAELMVERIFSFEVNVSVAIITFASQPKTIMSILSERSQDVTEVITSLDSASYKDHENATGTNTYEVLIRVYSMMQSQMDRLGMETSAWKEIRHTIILLTDGKSNMGDSPKKAVTRIRELLSIEQNRDDYLDIYAIGVGKLDVDWKELNELGSKKDGERHAFILQDAKALQQIFEHMLDVSKLTDTICGVGNMSANASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDIQMEDHHLWRVNVGDPTSQHGKEFLVEDVIIAPGFNVHAKRKQGISEFYADDIALLKLSRKVKMSTHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRLNINLRTGPEWTRCIQAVSQNKNIFPSLTNVSEVVTDQFLCSGMEEEDDNPCKGESGGAVFLGRRYRFFQVGLVSWGLFDPCHGSSNKNLRKKPPRGVLPRDFHISLFRLQPWLRQHLDGVLDFLPL

Molecular Weight

Approximately 90-110 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

C2/Complement C2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
C2/Complement C2 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74374
Quantity:
MCE Japan Authorized Agent: