CALM2 Protein, Human (His)

The CALM2 protein is an important component of calcium signaling, controlling enzymes, ion channels, and aquaporins through calcium binding. CALM2 Protein, Human (His) is the recombinant human-derived CALM2 protein, expressed by E. coli , with N-His labeled tag. The total length of CALM2 Protein, Human (His) is 149 a.a., with molecular weight of ~19 kDa.

CALM2 (Calmodulin 2) serves as a critical component in calcium signal transduction pathways, playing a pivotal role in the regulation of numerous enzymes, ion channels, aquaporins, and other proteins through its calcium-binding capabilities. The activation of calmodulin is contingent upon calcium binding, enabling it to exert control over various cellular processes. Notably, the calmodulin-calcium complex stimulates a range of enzymes, including myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), as well as phosphatases. CALM2, in conjunction with CCP110 and centrin, participates in a genetic pathway that governs the centrosome cycle and progression through cytokinesis. Additionally, CALM2 mediates calcium-dependent inactivation of CACNA1C and positively regulates the calcium-activated potassium channel activity of KCNN2. Furthermore, in the context of microbial infection, CALM2 is required for the arginine ADP-ribosanase activity of C.violaceum CopC. These multifaceted functions underscore CALM2's integral role in orchestrating cellular responses to calcium signals.

Human

E. coli

N-His

P0DP24 (M1-K149)

801  [NCBI]

MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.